After Mario defeated Master Thwomp once and for all in Mario in Mushroom Rix Land 3, peace returned to Rix Land. Mario settles in for a well-earned vacation on Mushroom Kingdom's beach. But Master Hand — childhood friend of both Master Thwomp and Bowser — is furious. He has been secretly building Nano Island, equipped with a deadly laser powerful enough to destroy both the Mushroom Kingdom and Rix Land. Mario gets the warning from Peach, Luigi, Yoshi, and the Toads, and immediately takes to the sky. Master Hand will not be ready for what is coming.