When a devastating energy weapon from deep space cripples Earth's defenses and a massive spacecraft emerges from the moon, Professor T. Bird realizes the Dark Queen has returned with an even deadlier scheme for galactic conquest. This time, however, she's far more cautious than before—enlisting the Shadow Boss as an unlikely ally to stack the odds overwhelmingly in her favor. The Battletoads trio of Zitz, Rash, and Pimple will need to contend not only with the Queen's own formidable roster of commanders and warriors, but also the Shadow Boss's brutal minions, forcing the amphibious heroes to accept unexpected aid. Recognizing the threat as an opportunity for vengeance, the Double Dragon brothers—Billy and Jimmy Lee—join forces with the Battletoads to even the playing field against their shared enemies and save the planet from invasion.