Genius Yamada — the mad scientist who was comprehensively defeated in the original Game Tengoku — is back. He has raided the You&Me Kamiyoga game center a second time and reasserted control over the video game world. Yuki Ito, the arcade clerk who helped beat him the first time, had a counter-program ready this time. JALECO's game characters mobilize once again. The situation is briefly complicated when Yuki is accidentally pulled into the game world by the same program she activated to fight Yamada.